DNase I | 合法醫療器材資訊網

ThermoScientificDNaseI,RNase-freeisanendonucleasethatdigestssingle-anddouble-strandedDNA.Ithydrolyzesphosphodiesterbondsproducingmono-​ ...

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.

In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.

Highlights

• Recombinant enzyme• Purified from non-animal host with a lower level of intrinsic RNases

Applications

• Preparation of DNA-free RNA• Removal of template DNA following in vitro transcription• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR• DNA labeling by nick-translation in conjunction with DNA Polymerase I• Studies of DNA-protein inter...