DNA Extraction Kit | 合法醫療器材資訊網

ThereareseveralmethodsforisolatingplasmidDNA.AtypicalDNAextractionkitusessomeformofalkalinelysisfollowedbyincubationwithaDNAbinding ...

As molecular biology techniques become more sensitive, it is increasingly important that a DNA extraction kit provides high-quality DNA. Techniques such as automated sequencing require high-purity DNA and minimal levels of contaminants, particularly inhibitory substances.

The key to an effective DNA extraction kit is the DNA-binding medium. The medium must strongly bind DNA on a large surface area to provide high yields. In addition, DNA binding media should exhibit weak binding to proteins and other molecules, so that proteins, RNA, and low molecular weight molecules can be eluted from the binding media under low-salt buffer conditions prior to elution of the DNA.

Unlike the extraction of genomic DNA, where all DNA is extracted, the isolation of plasmid DNA involves the separation of...