Bisulfite Sequencing of DNA | 合法醫療器材資訊網
由RPDarst著作·2010·被引用114次—Byselectiveconversionofcytosinebutnotm5Ctouracil,followedbyPCRandsequencingof...Thefivebasicstepsinbisulfiteconversionare:1)DNAdenaturation;...CAUTION:Seeabovewarnings(BASICPROTOCOL1
Curr Protoc Mol Biol. Author manuscript; available in PMC 2011 Nov 12.
Published in final edited form as:
PMCID: PMC3214597
NIHMSID: NIHMS217547
Department of Biochemistry and Molecular Biology and UF Shands Cancer Center Program in Cancer Genetics, Epigenetics and Tumor Virology, University of Florida College of Medicine
*To whom correspondence should be addressed. Michael P. Kladde, Ph.D., Tel: 352 273-8142; Fax: 352 273-8299; ude.lfu@eddalk[1] AbstractExact positions of ...
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Epigenetics | 合法醫療器材資訊網
Quick Guide of Popular Epigenetics Products. 品質保證 ... 利用bisulfite conversion,將non-methylated ... 附有control DNA 及primers,方便監控實驗流程。 Read More
Genome-wide | 合法醫療器材資訊網
由 J Reinders 著作 · 2008 · 被引用 81 次 — Methylation of cytosines (mC) is essential for epigenetic gene regulation in ... that bisulfite conversion followed by the “fourN” amplification produced material ... Non-treated and bisulfite-converted Columbia DNA Read More
Bisulfite Sequencing of DNA | 合法醫療器材資訊網
由 RP Darst 著作 · 2010 · 被引用 114 次 — By selective conversion of cytosine but not m5C to uracil, followed by PCR and sequencing of ... The five basic steps in bisulfite conversion are: 1) DNA denaturation; ... CAUTION: See above warnings (BASIC PROTOCOL 1 Read More
Bisulfite Conversion of DNA | 合法醫療器材資訊網
由 CA Leontiou 著作 · 2015 · 被引用 48 次 — Supplementary Materials. S1 Table: ... Introduction. Epigenetic alterations, including DNA methylation, play an important role in the regulation of gene expression. ... No. 59104, Qiagen), the MethylEdge Bisulfite C Read More
MethyLight and Digital MethyLight | 合法醫療器材資訊網
由 M Campan 著作 · 2018 · 被引用 7 次 — ... Methylation, Real-time PCR, TaqMan, Bisulfite, Epigenetics, Cancer, Quantitative, ... A threshold for bisulfite conversion quality control can be ... 2. Materials ... Highly purified DNA is not a requirement for Met Read More
Pyrosequencing Evaluation of Widely Available Bisulfite ... | 合法醫療器材資訊網
由 B Izzi 著作 · 2014 · 被引用 30 次 — Keywords: DNA methylationBisulfite treatmentPyrosequencing ... Since its original identification as an epigenetic regulator, numerous assays have ... Failed conversion occurs when an unmethylated cytosine is not deaminat Read More
Bisulfite Conversion Kit Manual | 合法醫療器材資訊網
whole or in part, without the prior written consent of Active Motif, Inc. This ... Active Motif's Bisulfite Conversion Kit simplifies analysis of DNA methylation by providing opti- ... control conversion-specific PCR primer pair can be used to assess the Read More
MethylEdge™ Bisulfite Conversion SystemPDF | 合法醫療器材資訊網
Methylation of cytosines in CpG dinucleotides is an epigenetic mechanism ... Bisulfite conversion of unmethylated cytosines to uracil. ... Use sterile, disposable, aerosol-resistant pipette tips and tubes. ... Materials to Be Supplied by the User ... Pre Read More
Bisulfite | 合法醫療器材資訊網
由 C Niemöller 著作 · 2021 — Niemöller et al. describe a bisulfite-free assay to assess epigenomics and ... was assessed by the analysis of non-methylated random spike-in control DNA ... with single-cell bisulfite sequencing, we show that cell-specific epi Read More
Whole genome amplification of sodium bisulfite | 合法醫療器材資訊網
由 J Mill 著作 · 2006 · 被引用 39 次 — Epigenetics refers to the heritable, but reversible, regulation of ... occasionally non-CpG sites] is one of the best-understood epigenetic ... After sodium bisulfite treatment, DNA regions of interest are amplified ... Read More
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【日祥重亞硫酸轉換品管物質 (未滅菌)】許可證字號:衛部醫器製壹字第007059號
器材品名:日祥重亞硫酸轉換品管物質(未滅菌)許可證字號:衛部醫器製壹字第007059號註銷狀態:註銷日期:註銷理由:有效日期:2022...