Monoclonal antibodies for use in an immunoradiometric assay ... | 合法醫療器材資訊網
由WMHunter著作·1982·被引用38次—...byamouseIgG1monoclonalantibodytohumanalpha-foetoprotein(AFP)forthepreparationof[125I]antibodyforuseinanimmunoradiometricassay(IRMA) ...
The advantages offered by a mouse IgG1 monoclonal antibody to human alpha-foetoprotein (AFP) for the preparation of [125I]antibody for use in an immunoradiometric assay (IRMA) have been investigated. The antibody was isolated from ascites fluid by sodium sulphate precipitation followed by gel filtration on Sephadex G-200. The freeze-dried powder and solutions thereof were stable and were used for iodination to 1 atom 125I/molecule antibody by the chloramine-T procedure. At high antigen concentrations 70-80% of the added [125I]Ab was present in the sandwich. Linear response curves in the range 1-100 micrograms antigen/l incubate were obtained when [125I]Ab was in slight excess. In this region an Ag : Ab ratio of 1.9 : 1 was obtained which is consistent with the saturation of a bifunctional antibody. Although non-specific binding (in the absence of antigen) was consistently less than 0.1% of added [125I]Ab, this was t...
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Monoclonal antibodies for use in an immunoradiometric assay ... | 合法醫療器材資訊網
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【常盈甲型胎蛋白放射免疫檢驗試劑】許可證字號:衛署醫器輸字第021107號
器材品名:常盈甲型胎蛋白放射免疫檢驗試劑許可證字號:衛署醫器輸字第021107號註銷狀態:已註銷註銷日期:2018/04/25註銷理由:...