CISH Tips | 合法醫療器材資訊網
![CISH Tips](https://i.imgur.com/6y29Lx7.jpg)
Chromogenicinsituhybridization(CISH)isusedtodetectspecificnucleicacidsequences...Invitrogen'spatentedSPT(SubtractionProbeTechnology)utilizesan...OnecommonquestionthatarisesiswhetherornotSPOT-Light®probesworkwith...
![CISH Tips](https://i.imgur.com/6y29Lx7.jpg)
For CISH with cell preparations, the pretreatment step is different than for FFPE sections. With cells, we recommend omitting the heat treatment step, but using the pepsin enzyme treatment for antigen retrieval. The remainder of the protocol for CISH with FFPE is the same. For cell or metaphase chromosome samples, we recommend the following modified CISH protocol:
1) Immerse the slides in 2x SSC buffer for 60 minutes at 37°C.
2) (Optional) Pretreat cells with SPoT-Light™ Cell Pretreatment Reagent (Cat. No. 00-8402) for 5 min at 37°C. Variations in incubation time (2-10 min) may be required, depending on the cell type and how the slides are made. Remember that over-digestion with pepsin will cause loss of nuclei and chromosome structure, while inadequate digestion may result in loss of signal.
3) Wash the slides three times in distilled water at room temperat...
*1* invitrogen | 合法醫療器材資訊網
CISH Tips | 合法醫療器材資訊網
CISH Tips | 合法醫療器材資訊網
CISH Tips | 合法醫療器材資訊網
CISH Tips | 合法醫療器材資訊網
CISH Tips | 合法醫療器材資訊網
SPoT | 合法醫療器材資訊網
SUMMARY OF SAFETY and EFFECTIVENESS (SSED) | 合法醫療器材資訊網
Zymed (IVGN) SPOT | 合法醫療器材資訊網
![](https://i.imgur.com/6y29Lx7.jpg)
【"萊富生命" 聚焦原位雜交高分子偵測試劑組】許可證字號:衛署醫器輸壹字第008127號
器材品名:"萊富生命"聚焦原位雜交高分子偵測試劑組許可證字號:衛署醫器輸壹字第008127號註銷狀態:已註銷註銷日期:2018/06/25...