Identification of cell surface markers and establishment of ... | 合法醫療器材資訊網
由APReyes著作·2020·被引用7次—Invitrodifferentiationofhumanpluripotentstemcellsintofunctionalretinal...weperformacomprehensiveantibodyscreeningandidentifycellsurfacemarkersforRPEcells.Weshowthatthesemarkerscanbeused
Human cell surface marker screeninghESC and hPSC-RPE cells were dissociated into single cells using TrypLE for 5–10 min. Optic vesicles were dissociated into single cells using TrypLE Select (Gibco, Invitrogen) for 10 min, followed by physical dissociation through a 20 G needle. To allow the simultaneous analysis of these different populations in the same sample, hESC, hPSC-RPE cells, and optic vesicle cells were labeled with CellTrace™ CFSE (0.25 µM) for 7 min at 37 °C or CellTrace™ Violet (5 µM) for 20 min at 37 °C following the manufacturer’s protocol (Thermo Fisher Scientific). Then, the three cell types were stained using the BD Lyoplate™ Screening Panels (BD Biosciences) following the manufacturer’s protocol. The barcoding of cells allowed to distinguish easily between the three groups of cells and to minimize sample variability during the screening. Samples were analyzed on 96-well plates on ...
(PDF) Immunophenotyping of leukemias using a cluster of ... | 合法醫療器材資訊網
Development of Novel Monoclonal Antibodies that Define ... | 合法醫療器材資訊網
Leucocyte common antigen (CD45) and CD5 positivity in an ... | 合法醫療器材資訊網
Porcine cluster of differentiation (CD) markers 2018 update | 合法醫療器材資訊網
Genomically | 合法醫療器材資訊網
Identification of cell surface markers and establishment of ... | 合法醫療器材資訊網
Human CD antigen chart | 合法醫療器材資訊網
Immunophenotyping of Leukemias Using a Cluster of ... | 合法醫療器材資訊網
CD Marker Panel | 合法醫療器材資訊網
【諾法卡特細胞CD標誌系列抗體】許可證字號:衛署醫器輸壹字第004088號
器材品名:諾法卡特細胞CD標誌系列抗體許可證字號:衛署醫器輸壹字第004088號註銷狀態:已註銷註銷日期:2013/01/18註銷理由:許...