Fluorescence In Situ Hybridization (FISH) protocol | 合法醫療器材資訊網

Fluorescenceinsituhybridization(FISH)isatechniquethatusesfluorescentprobeswhichbindtospecialsitesofthechromosomewithahighdegreeof ...

FISH Principle

Fluorescence in situ hybridization (FISH) is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes. The fluorescent probes are nucleic acid labeled with fluorescent groups and can bind to specific DNA/RNA sequences. Thus, we can understand where and when a specific DNA sequences exist in cells by detecting the fluorescent group. It was developed in the early 1980s. Fluorescence microscopy can be used to find out where the fluorescent probe is bound to the chromosomes and flow cytometry can be used to detect the binding quantitatively. This FISH protocol is for a Cy5 and FAM labeled probe used in flow cytometry detection and fluorescence microscopy detection.