Fluorescence In Situ Hybridization (FISH) | 合法醫療器材資訊網

Fluorescenceinsituhybridization(FISH)isalaboratorytechniquefordetectingandlocatingaspecificDNAsequenceonachromosome.

Fluorescence in Situ Hybridization (FISH). Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that allows the localization of a specific DNA sequence or an entire chromosome in a cell. It is utilized to diagnose genetic diseases, gene mapping, and identification of chromosomal abnormalities, and may also be used to study comparisons among the chromosomes arrangements of genes of related species. FISH involves unwinding of the double helix structure and binding of the DNA of all probes attached to a fluorescent molecule with a specific sequence of sample DNA, which can be visualized under the fluorescent microscope.