Why nanopore technology? | 合法醫療器材資訊網

StratosGenomics,aRochecompany,hasdevelopedanovelchemistrycalledSequencingbyeXpansion(SBX)tosignificantlyimprovetheaccuracyandthroughputof ...

Stratos Genomics, a Roche company, has developed a novel chemistry called Sequencing by eXpansion (SBX) to significantly improve the accuracy and throughput of nanopore sequencing. This chemistry translates the sequence of DNA into a simple to measure surrogate molecule called an Xpandomer. Much like with polymerase chain reaction (PCR), Xpandomer synthesis is based on the natural function of DNA replication where expandable nucleoside triphosphates (X-NTPs) act as substrates for template-dependent, polymerase-based replication.

Xpandomer synthesis is based on four easily differentiated X-NTPs (also called High Signal-to-Noise Reporters), one for each DNA base. Engineered polymerases incorporate these modified nucleotides into Xpandomers, exactly copying the target nucleic acid template from the library. As the Xpandomer molecule transits through the nanopore, the distinct electrical signal of each ...