Behind the Coulter Principle | 合法醫療器材資訊網

WiththeCoulterPrinciple,particlesorcellspassingthroughanelongatedglasstubewithafinely-boredrubydisk(aperture)designedtoconstrictthe ...

With the Coulter Principle, particles or cells passing through an elongated glass tube with a finely-bored ruby disk (aperture) designed to constrict the flow of fluid through the tube while also constricting the current to a controlled value with little variation.

When an aperture is placed between two electrodes and a current path is introduced by a low-concentration electrolyte, the resistance between the electrodes can be measured. This is called the sensing zone. As a particle passes through the aperture, a volume of electrolyte-containing solution equivalent to the immersed volume of the particle is displaced from the sensing zone causing a short-term change in impedance across the aperture which can be measured as a voltage or current pulse. The voltage pulse is proportional to the volume of the detected particle.

Using counter and pulse analyzer circuits, the number and volume o...