DNA Quantification using Gen5™ | 合法醫療器材資訊網

Pathlength Correction. In microplate readers (unlike standard cuvette spectropho- tometers), the path of light through the sample varies with. Read More

Pathlength correction normalizes all the wells to a common pathlength, which is directly related to volume. This is demonstrated in Figure 3, where samples with ... Read More

2012年12月12日 — BioTek Technical Notes, 12-Dec-12, DNA Quantification using Gen5™ ... Sample measurements can then be path length corrected by dividing them ... Read More

ments are equivalent to traditional 1 cm pathlength measurement methods. ... BioTek s microplate readers. The monochromator- ... Backgrounds were corrected. Read More

2013年1月31日 — Since the sample volume can vary in microplate format, a path length correction factor must be applied to standardize results to that of a 1 ... Read More

... Agilent BioTek Gen5 stware can facilitate assay miniaturization to microplates by providing optional cuvette ports and automated pathlength correction. Read More

Here we describe methodology to quantitate nucleic acids using the BioTek PowerWave™ ... the absorbance of each well at the selected pathlength correction ... Read More

Figure 3. Pathlength Correction in 384-well Microplates. Volumes ranging from 10 to 100 l of DNA samples (100 g/ml) were aliquoted into 384 ... Read More

BioTek's reader control and data analysis software, Gen5™, has built-in methods for path length correction to 1 cm for samples in microplates where path length ... Read More

2015年3月13日 — Pathlength correction for plate readers applies to microplates, in which the pathlength is the height of the liquid in the well. Cite. Read More