Upright and Inverted Microscopy | 合法醫療器材資訊網

Invertedmicroscopyisaverypopulartechniqueforlivecellimaging.Here,livingcellsareobservedthroughthebottomofacellculturevessel.

In cell biology, upright microscopes are used for phase contrast or widefield fluorescence microscopy of living cells or samples that are squeezed between a slide and coverslip. An additional application is the microscopy of fixed cells or tissue sections.

In principle, upright microscopes can be used for live cell imaging, especially with dipping objective lenses. This type of system is often used for larger samples like tissue and animals.

However, there are several disadvantages of upright microscopes when compared to inverted microscopes. In general, the working distances in upright microscopes are longer, which leads to lower resolution and weaker fluorescence signals. In contrast to inverted microscopes, upright microscopes do not allow for the use of objectives with high numerical aperture directly on the coverslip bottom, on whic...