MicroRNA Experimental Protocols | 合法醫療器材資訊網
Regularreal-timequantitativepolymerasechainreaction(PCR)canonlybeusedtodetectmiRNAprecursors,whilestem-loopreal-timequantitativeRTPCR ...
Overview
MicroRNAs (miRNAs) are non-coding small RNAs, suppressing gene expression or inhibiting translation by binding (sometimes partially) complementary sequences in the 3 UTR of mRNAs. Recent studies indicate that microRNA-AGO complex, when paired with an unusual mRNA, promotes microRNA decay rather than mRNA degradation [3]. MicroRNAs regulate biological processes including cell proliferation, apoptosis, differentiation, metabolism, development, neurogenesis [4], neuronal functions [5, 6] and neoplastic transformation [7, 8]. MicroRNAs have been proposed as diagnostic markers [9] and the therapeutical potentials of microRNA mimics are being tested [10]. MiRNA genes are encoded in intergenic chromosomal regions or within the introns of protein-coding genes. Most recently, miRNAs have been shown to originate from protein-coding gene promoters with unique features [11]. MicroRN...
Manual | 合法醫療器材資訊網
microRNA & Noncoding RNA Analysis Using Real | 合法醫療器材資訊網
MicroRNA Analysis Using TaqMan Assays | 合法醫療器材資訊網
MicroRNA Experimental Protocols | 合法醫療器材資訊網
Protocol: a highly sensitive RT-PCR method for ... | 合法醫療器材資訊網
Protocol | 合法醫療器材資訊網
qPCR | 合法醫療器材資訊網
TaqMan MicroRNA Assays Protocol | 合法醫療器材資訊網
TaqMan MicroRNA Assays | 合法醫療器材資訊網
TaqMan™ MicroRNA Assay | 合法醫療器材資訊網
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