RNase A in TNE | 合法醫療器材資訊網

RNaseAinTNE.RNaseA(RocheAppliedScience).cautionTris-Cl(10mM,pH7.5)/NaCl(15mM).cautionTNEforSISH.Topreparea10mg/mLRNaseAstock ...

RNase A (Roche Applied Science)

Tris-Cl (10 mM, pH 7.5)/NaCl (15 mM)

TNE for SISH

To prepare a 10 mg/mL RNase A stock solution, dissolve 100 mg of RNase A in 10 mL of Tris-Cl (10 mM, pH 7.5)/NaCl (15 mM). Heat to 100ºC for 5 min and cool at room temperature. Store at −20ºC.

For a working dilution of 2 μg/mL, mix 20 μL of RNase A stock solution with 100 mL of TNE for SISH. Use immediately.