Silver Staining- Principle, Procedure | 合法醫療器材資訊網
2020年9月16日—Principleofsilverstaining·Thismethodusesadiaminecomplexofsilvernitrateinanalkalinespacemadeupofammoniumandsodiumhydroxide ...
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What is silver staining?Silver staining is a special yet powerful staining technique that is used for the detection and identification of proteins[1] in gels.
This is because silver binds to the chemical terminal or side chains of amino groups i.e carboxyl and sulfhydryl groups. It has been used for decades now to separate proteins from polyacrylamide gel electrophoresis. The nucleation sites where there are tiny crevices where the free gas-liquid surface is maintained in proteins, promote formaldehyde reduction of silver ions into microscopic silver crystals which facilitate their detection. The protein detection by silver staining is a highly sensitive method yet specific and selective for proteins. It produces an image with reduced background and less mass spectrometry interference. The general procedure for silver staining includes the fixation of silver, sensitization, impregnation of silver, and development of an image. Several var...
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Principle and Method of Silver Staining of Proteins ... | 合法醫療器材資訊網
Principle and Method of Silver Staining of Proteins Separated ... | 合法醫療器材資訊網
The technique is based on the simple principle that selective reduction of silver into metallic silver occurs at the initiation site in the close proximity of ... Read More
Protein Gel Staining Methods | 合法醫療器材資訊網
General principles of gel staining — General in-gel protein staining protocol involving ... For example, silver staining requires both a staining ... Read More
Silver staining of proteins in polyacrylamide gels | 合法醫療器材資訊網
由 M Chevallet 著作 · 2006 · 被引用 572 次 — Unlike staining with organic dyes, silver staining goes against general thermodynamics. Proteins bind silver ions, and this binding decreases ... Read More
Silver Staining- Principle, Procedure | 合法醫療器材資訊網
2020年9月16日 — Principle of silver staining · This method uses a diamine complex of silver nitrate in an alkaline space made up of ammonium and sodium hydroxide ... Read More
Silver staining of proteins in polyacrylamide gels | 合法醫療器材資訊網
由 M Chevallet 著作 · 2006 · 被引用 572 次 — Unlike staining with organic dyes, silver staining goes against general thermodynamics. Proteins bind silver ions and this binding decreases the reactivity of ... Read More
Silver Staining Protocol | 合法醫療器材資訊網
2019年6月30日 — Protocol (Fernandez-Patron C. et al. 1992) · Rinse the gel with water for 5-10 minutes. · Soak the gels in 20% (v/v) ethanol containing 10% (v/v) ... Read More
Silver staining | 合法醫療器材資訊網
In traditional stained glass, silver stain is a technique to produce yellow to orange or brown shades (or green on a blue glass base), by adding a mixture ... Read More
1 Silver staining of proteins in polyacrylamide gels | 合法醫療器材資訊網
由 T Rabilloud 著作 · 2009 · 被引用 288 次 — The simple application of these two principles in a silver staining protocol is outlined in figure 1 an results in negative staining, as shown by Merril's group ... Read More
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【東耀帕氏染色液】許可證字號:衛署醫器製壹字第002269號
器材品名:東耀帕氏染色液許可證字號:衛署醫器製壹字第002269號註銷狀態:註銷日期:註銷理由:有效日期:2018/10/23發證日期:200...