Stain,Buffer,(BSA),RUO - 554657 | 合法醫療器材資訊網

BrandBDPharmingen™;ApplicationFlowcytometry(RoutinelyTested)...Sizes500mL;CatalogNo.554657.UnitEA(1Each).Quantity.Addtoquote.Saveto ...

Procedure for Using Pharmingen Stain Buffer (BSA) for the Direct Immunofluorescent Staining of Cells.

1.        Prepare single-cell suspensions from either lymphoid tissue, bone marrow, peripheral blood or cell cultures using standard protocols.

2.        Wash the cells twice in cold Pharmingen Stain Buffer (BSA) and pellet the cells by centrifugation (e.g., 300 x g at 4°C). Resuspend the cell

        pellet with cold Pharmingen Stain Buffer (BSA) to a final concentration of 2 x 10e7 cells/ml.

3.        Distribute 50 µl aliquots of the cell suspension (10e6 cells) to either tubes or the round-bottomed wells of microwell plates.

4.        Dilute flu...