Direct flow cytometry (FACS) protocol | 合法醫療器材資訊網

Generalprocedureforflowcytometryusingaconjugatedprimaryantibody.·Harvest,washthecells,andadjustcellsuspensiontoaconcentrationof1-5x106 ...

General procedure for detecting intracellular or extracellular proteins in flow cytometry[1].

Updated July 17, 2023.

1: Sample preparation

First, we should harvest our cells or tissue and prepare a single-cell suspension. We will then transfer our single-cell suspension into 96-well plates, test tubes, or polystyrene round bottom tubes, depending on the number of cells and volumes being used.

Materials required:

• Cell suspension 

• Polystyrene round bottom 12 x 75 mm2 Falcon tubes/96-well plate (or any container compatible with your centrifuge)

• Suspension/washing buffer (PBS, 5-10% fetal cell serum (FCS))

• Optional – red blood cell lysis buffer (example ab204733)

Time needed =20 minutes approx

First, we should harvest our cells or t...