Composition of commercial media used for human embryo ... | 合法醫療器材資訊網

由DEMorbeck著作·2014·被引用113次—Culturemediacompositionvarieswidely,withdifferencesinpyruvate,lactate,andaminoacidsespeciallynotable.Blastocystdevelopmentwasculturemedia ...

Objective: To determine the composition of commercially available culture media and test whether differences in composition are biologically relevant in a murine model.

Design: Experimental laboratory study.

Setting: University-based laboratory.

Animal(s): Cryopreserved hybrid mouse one-cell embryos were used in experiments.

Intervention(s): Amino acid, organic acid, ions, and metal content were determined for two different lots of media from Cook, In Vitro Care, Origio, Sage, Vitrolife, Irvine CSC, and Global. To determine whether differences in the composition of these media are biologically relevant, mouse one-cell embryos were thawed and cultured for 120 hours in each culture media at 5% and 20% oxygen in the presence or absence of protein in an EmbryoScope time-lapse incubator.

Main outcome measure(s): The compositions of seven...