Western blot sample preparation | 合法醫療器材資訊網

Discovervarioussamplepreparations,includinglysisbuffers,lysatefromcell...RIPAbufferisusefulforwholecellextractsandmembrane-boundproteins,and ...

Lysis buffers differ in their ability to solubilize proteins, with those containing sodium dodecyl sulfate (SDS) and other ionic detergents considered to be the harshest and therefore most likely to give the highest yield.

The main consideration when choosing a lysis buffer is whether the chosen antibody will recognize denatured samples. When this is not the case, it will be noted on the antibody datasheet, and buffers without detergent or with relatively mild non-ionic detergents (NP-40, Triton X-100) should be used.  ​