Flow Cytometry Protocol for Analysis of Cell Viability using ... | 合法醫療器材資訊網

ThefollowingprotocolhasbeendevelopedandoptimizedbyR&DSystemsFlowCytometryLaboratoryforcellviabilitystainingusingpropidiumiodide.

Propidium iodide (PI)[1] is a membrane impermeant dye that is generally excluded from viable cells. It binds to double stranded DNA by intercalating between base pairs. PI is excited at 488 nm and, with a relatively large Stokes shift, emits at a maximum wavelength of 617 nm. Because of these spectral characteristics, PI can be used in combination with other fluorochromes excited at 488 nm such as fluorescein isothiocyanate (FITC)[2] and phycoerythrin (PE)[3].

Propidium iodide (PI) staining is a viability dye flow cytometry method used to assess cell viability. PI staining can provide information about the cell cycle and DNA content of the population. This information is valuable in translational research, particularly in the field of oncology, where PI staining can be used to assess the efficacy of anticancer drugs....