MALDI-TOF mass spectrometry | 合法醫療器材資訊網

由NSinghal著作·2015·被引用841次—However,thedevelopmentofelectronsprayionization(ESI)andmatrixassistedlaserdesorptionionization(MALDI)in1980sincreasedthe ...

The genomic information within a microbial cell translates into more than 2000 proteins, a substantial number of which can be studied using proteomics (Wasinger et al., 1995[1]). It is estimated that for genomes which contain less than 1000 genes, more than 50% of predicted proteome may be identified from the genome. Similarly 30 and 10% predicted proteome may be identified from genomes which carry ca. 2500 and ca. 4000 genes respectively (Jungblut and Hecker, 2004[2]). Thus, a microbial genome containing 600–7000 predicted genes represents a medium-sized complex system where application of proteomics may provide knowledge of a substantial part of the microbe’s proteome.

Characterization and differentiation of microbial proteome developed and progressed with both gel-based and gel-free protein separation methods. SDS-PAGE of whole cell proteins, ...