Plasmid Isolation | 合法醫療器材資訊網

Plasmid isolation[1] is crucial to biology and an essential step in various procedures, including cloning, DNA sequencing, transfection, in vitro translation, blotting, and gene therapy. However, these applications require the isolation of high-purity plasmid DNA. Whereas genomic DNA extraction is simple and straightforward, plasmid DNA extraction can be more complicated.

In plasmid isolation, you must be able to identify and use the appropriate lysis method to extract plasmid DNA successfully. Therefore, you must use a milder treatment such as alkaline lysis. Alkaline lysis is a mild treatment and the preferred method of plasmid DNA isolation and extraction. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation.