Southern blot trouble | 合法醫療器材資訊網

2015年11月5日 — Southern blot troubleshooting: no signal? Hi there,. I am using Southern blotting to detect telomeres in my samples and have been following a ... Read More

I use the Roche DIG Reagents to the Southern-Blot with DNA Probes labeled in DIG, revelation with anti-Digoxigenin (alkaline phosphatase) and CDP Star. Read More

Too low amounts of gDNA is the most common cause, why Southern Blotting does not give signals. Always be aware, that for all 3 methods mentioned above ... Read More

The blot looks exactly the same as the gel transferred onto it and it appears to be overcooked or have a lot of background issues occurring. For the second attempt,​ ... Read More

Another way to troubleshoot the Southern blotting procedure is to use a probe known to work on bacterial genomic DNA. This can be done conveniently by slicing ... Read More

Blot I : Name THREE possible problems that could cause this (blank blot, no bands). 1. Failure of DNA to transfer to membrane. 2. Forgot to bake membrane &​ ... Read More

No signal in southern blot - troubleshooting (reply: 2); Help! Campylobacter Southern Blotting problems - can't get any signal from blot, should i try a different kit? Read More

2012年5月30日 — Troubleshooting Southern Blots ... There are several problems that may occur that will result in the failure of a signal to appear on the final blot. To ... Read More

Troubleshooting — For troubleshooting of the process the following steps can be ... PCR technology has replaced the Southern Blotting technique. Read More

由 E LOTTIN 著作 · 1987 — support tray. Figure 2.9.1 Transfer pyramid for Southern blotting. ... Problems with the probe are detectable by straightfor- ward measurement of incorporation of. Read More