The Basics: RNA Isolation | 合法醫療器材資訊網

ThetraditionalmethodforassessingRNAconcentrationandpurityisUVspectroscopy.TheabsorbanceofadilutedRNAsampleismeasuredat260and280nm.

Obtaining high-quality RNA is the first, and often the most critical, step in performing many molecular techniques such as reverse transcription real-time PCR (RT-qPCR), transcriptome analysis using next-generation sequencing, array analysis, digital PCR, northern analysis, and cDNA library construction. To generate the most sensitive and biologically relevant results, the RNA isolation procedure must include some important steps before, during, and after the actual RNA purification. The following application note discusses various best practices in sample collection, storage, and RNA extraction procedures for maximizing the yield and quality of sample RNA, many of which involve the use of Invitroge...