Plasmid Isolation | 合法醫療器材資訊網

Thebasicstepsofplamidisolationaredisruptionofthecellularstructuretocreatealysate,separationoftheplasmidfromthechromosomalDNA,celldebrisand ...

The term ‘plasmid’ was coined by Joshua Lederberg in 1952. Originally evolved from bacteria, plasmids are extrachromosomal genetic elements present in most species of Archae, Eukarya and Eubacteria that can replicate independently. Plasmids are circular double stranded DNA[1] molecule that are distinct from the cells chromosomal DNA.

The structure and function of a bacterial[2] cell is directed by the genetic material contained within the chromosomal DNA. In some cases plasmids are generally not essential for the survival of the host bacterium. Although not essential, plasmids contribute significantly to bacterial genetic diversity and plasticity by encoding functions that might not be specified by the bacterial chromosomal DNA. Plasmids specify traits that allow the host to persist in environments that would otherwise be either lethal or restrictive for growth. For exa...