Methods for extracting genomic DNA from whole blood ... | 合法醫療器材資訊網

由DChacon-Cortes著作·2014·被引用83次—Aspreviouslymentioned,lysisofcellsisacommonstepinmostDNAextractionprotocols,anditiscommonlyachievedthroughtheuseof ...

Genomics Research Centre, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, QLD, Australia

Abstract: Deoxyribonucleic acid (DNA) extraction has considerably evolved since it was initially performed back in 1869. It is the first step required for many of the available downstream applications used in the field of molecular biology. Whole blood samples are one of the main sources used to obtain DNA, and there are many different protocols available to perform nucleic acid extraction on such samples. These methods vary from very basic manual protocols to more sophisticated methods included in automated DNA extraction protocols. Based on the wide range of available options, it would be ideal to determine the ones that perform best in terms of cost-effectiveness and time efficiency. We have reviewed DNA extraction history and the most commonly used methods for DNA extraction from whole blood samples...