The Basics: RNase Control | 合法醫療器材資訊網

AbouttheRNaseenzyme...RNases,especiallythosebelongingtotheRNaseAfamily,arefairlysmall,compactproteinscontainingseveralcysteineresiduesthat​ ...

Common sources of RNase contamination include lab benches, pipettors, glassware, tubes, tips, and electrophoresis equipment. When working with RNA, it’s important to ensure that lab plastic consumables, such as microcentrifuge and PCR tubes and pipette tips, are RNase-free. All lab surfaces, such as benchtops, centrifuges, and electrophoresis equipment should be presumed contaminated with RNases since they are exposed to environmental contaminants. Sources of RNase contamination on lab surfaces often include bacterial and fungal spores and dead cells shed from human skin (e.g., hands).

Due to the ubiquitous nature of RNases, water and buffers used in molecular biology applications can be frequent sources of RNase contamination. DEPC treatment is the most common method used to inactivate RNases in water and buffers. However, certain reagents such as Tris cannot be DEPC-treated.

Though RNas...