Bradford protein assay | 合法醫療器材資訊網

TheBradfordproteinassaywasdevelopedbyMarionM.Bradfordin1976....Itisaquickandaccuratespectroscopicanalyticalprocedureusedtomeasurethe ...

Method to determine protein concentration

The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976.[1] It is a quick and accurate[2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.

The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie brilliant blue G-250. The Coomassie brilliant blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red).[3] Under acidic conditions, the red form of the dye is converted into its...