ELISA | 合法醫療器材資訊網

Theassayusesasolid-phasetypeofenzymeimmunoassay(EIA)todetectthepresenceofaligand(commonlyaprotein)inaliquidsampleusingantibodies ...

Method to detect an antigen using an antibody and enzyme

The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971.[1] The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.

In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are removed. In the final step, a substance containing the enzymes substrate is add...