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Protocol | 合法醫療器材資訊網

Protocol

Apply100µlDABsubstratesolution(freshlymadejustbeforeuse:0.05%DAB-0.015%H2O2inPBS)tothesectionsontheslidestorevealthecolorofthe ...

 Protocol

 

Introduction

 

The following is a general procedure guide for preparation and staining of acetone-fixed frozen tissues using a purified, unconjugated primary antibody, biotinylated secondary antibody and streptavidin-horseradish peroxidase (SAv-HRP) and DAB detection system. Because each antigen differs in terms of requirement for fixation, amplification step, etc., it is not possible to write an inclusive protocol that will work for all antigens. The user must determine optimal conditions for each antigen of interest. Many protocols for staining individual antigens, as well as useful tips and troubleshooting guides for immunohistochemistry, can be found at the IHC World web site (https://www.ihcworld.com/[1]).

  Protocol Steps

 

Prepare frozen tissue sections (steps 1-8):

 

Place a...

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Protocol | 合法醫療器材資訊網
Apply 100 µl DAB substrate solution (freshly made just before use: 0.05% DAB - 0.015% H2O2 in PBS) to the sections on the slides to reveal the color of the ... Read More
IHC protocol: paraffin | 合法醫療器材資訊網
Paraffin and frozen sections · Apply enzyme-conjugated secondary antibody to the slide, diluted in TBS with 1% BSA, and incubate for 1 h at room temperature. Read More
IMMUNOHISTOCHEMISTRY (IHC-FR) | 合法醫療器材資訊網
Continue with the immunohistochemical staining protocol. The absence of formalin eliminates the need for an antigen retrieval step. However, if frozen tissue or ... Read More
Protocol for the Preparation and Fluorescent IHC Staining of ... | 合法醫療器材資訊網
For fluorescent IHC staining of frozen tissue sections using R&D Systems antibodies, it is recommended to incubate overnight at 2-8 °C. This incubation regime ... Read More
Immunohistochemistry staining of frozen sections ... | 合法醫療器材資訊網
(IHC-Fr) frozen sections. This protocol is a general guide for formaldehyde-based fixation, cryostat sec廿oning， and fluorescent staining of frozen tissue ... Read More
Immunohistochemistry Frozen Protocol | 合法醫療器材資訊網
Fluorescent IHC Staining of Frozen Tissue · Fluorescent Immunohistochemistry/​Immunofluorescence is a technique which utilizes fluorescently labeled antibodies ... Read More
Immunohistochemistry Frozen Troubleshooting | 合法醫療器材資訊網
Read and print our Immunohistochemistry Frozen Protocol. Tips, techniques and troubleshooting available to help with your experiments! Read More
BestProtocols | 合法醫療器材資訊網
eBioscience Best Protocols: immunohistochecmistry (IHC) staining of frozen tissue (indirect method) using purified antibody. Read More
BestProtocols | 合法醫療器材資訊網
eBioscience Best Protocols: immunohistochecmistry (IHC) staining of frozen tissue (direct method). Read More
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