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Bloodagarcontainsgeneralnutrientsand5%sheepblood.Itisusefulforcultivatingfastidiousorganismsandfordeterminingthehemolyticcapabilitiesofan ...

Blood Agar

Blood agar contains general nutrients and 5% sheep blood.  It is useful for cultivating fastidious organisms and for determining the hemolytic capabilities of an organism. 

Some bacteria produce exoenzymes that lyse red blood cells and degrade hemoglobin; these are called hemolysins. Bacteria can produce different types of hemolysins.  Beta-hemolysin breaks down the red blood cells and hemoglobin completely. This leaves a clear zone around the bacterial growth. Such results are referred to as β-hemolysis (beta hemolysis). Alpha-hemolysin partially breaks down the red blood cells and leaves a greenish color behind.  This is referred to as α-hemolysis (alpha hemolysis).  The greenish color is caused by the presence of biliverdin, which is a by-product of the breakdown of hemoglobin.  If the organism does not pro...