Effects of Different Cell | 合法醫療器材資訊網

由KTsuji著作·2017·被引用74次—TrypsinandTrypLEquicklydissociatedthecellswithin5min,while...LCA,leukocytecommonantigen;ITGA3,integrinα3;ITGAV,integrinαV; ...

To examine if the reduction of CD73, CD105, and CD140b expression by long-term trypsin treatment altered stem cell character irreversibly, cells subjected to detachment by trypsinization for 60 min were reseeded and cultured for another 14 days under normal conditions (), and the expression of these antigens was analyzed. As shown in , the reduced expression of CD73, CD105, and CD140b by long-term trypsin treatment (60 min) was completely reversed by recovery culture. Immunohistochemical (, left) and flow cytometric analyses (, right) indicated that the expression of CD73, CD105, and CD140b quickly reversed by day 3 (CD73 and CD105) or by day 5 (CD140b). These data indicate that the loss of the expression of these surface antigens by long-term trypsin digestion might not induce an irreve...