KAPA HiFi HotStart Real | 合法醫療器材資訊網

Description·High-fidelityPCRisusedtoselectivelyenrichlibraryfragmentscarryingappropriateadaptersequencesandtoamplifytheamountofDNApriorto ...

KAPA HiFi HotStart Real-Time Library Amplification Kits are designed for the amplification of NGS libraries prepared for Illumina sequencing.

KAPA HiFi HotStart Real-Time Library Amplification Kits contain KAPA HiFi HotStart Real-Time PCR Master Mix (2X), a ready-to-use cocktail containing all components for PCR, except primers and template. The master mix contains KAPA HiFi HotStart DNA Polymerase in a proprietary reaction buffer, dNTPs, MgCl2 (2.5 mM at 1X), SYBR® Green I dye, and stabilizers. Four fluorescent standards are supplied and are used to define a window for optimal amplification

High-fidelity PCR is used to selectively enrich library fragments carrying appropriate adapter sequences and to amplify the amount of DNA prior to sequencing. During PCR enrichment of libraries, minimizing amplification bias is critical to ensure uniform sequence coverage. Amplification bias occurs ...