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DNA Extraction and Purification | 合法醫療器材資訊網

DNA Extraction and Purification

ThebasiccriteriathatanymethodofDNAisolationfromanysampletypeshouldmeetinclude:(1)efficientextractionofDNAfromthesample,(2)productionofa​ ...

 DNA Extraction and Purification

Introduction

Basic considerations

DNA extraction is required for a variety of molecular biology applications. Figure 1 lists the basic steps involved in all DNA extraction methods. Many commercial kits are available to isolate DNA from a variety of biological materials [1, 2]. The sensitivity of polymerase chain reaction (PCR) detection has been shown to be different for various DNA kits [3]. Therefore, selecting the best methodology for your application is crucial.

Figure 1. Basic steps involved in all DNA extraction methods.

Choosing the correct DNA extraction kit can save crucial time on optimization and execution of the experiment. Factors to be considered for selecting a kit include:

Sample origin: Different kits are used to extract material from specific sources, including human tissues, blood, hair, rodent tissues, leaf tissue, bacteria, yeast, fungi, insect, stool, body fluids, spores, soil, clinical samples (e.g., biopsy samples, fine nee...

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DNA | 合法醫療器材資訊網
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DNA Extraction Protocols | 合法醫療器材資訊網
A collection of DNA Extraction Protocols for research, provided by Invitrogen. Read More
DNA Extraction from Tissue | 合法醫療器材資訊網
Protocol - Isolating Genomic DNA Using the Micro Tissue Kit — The protocol recommends eluting the genomic DNA in 150 µl (Micro Kit) or 250 ... Read More
Genomic DNA Extraction | 合法醫療器材資訊網
Purification Procedure — If none of the sample preparation protocols match the type or size of your sample, then use the guidelines described below to ... Read More
DNA Extraction Protocols | 合法醫療器材資訊網
A collection of DNA Extraction Protocols for research, provided by Invitrogen. Read More
Quick Protocol for Extraction and Purification of Genomic DNA ... | 合法醫療器材資訊網
2019年4月30日 — Incubate at 56°C in a thermal mixer with agitation at full speed (1400 rpm) until tissue pieces have completely dissolved (typically 30-60 minutes). Read More
DNA Extraction and Purification | 合法醫療器材資訊網
The basic criteria that any method of DNA isolation from any sample type should meet include: (1) efficient extraction of DNA from the sample , (2) production of a​ ... Read More
Methods for extracting genomic DNA from whole blood ... | 合法醫療器材資訊網
由 D Chacon-Cortes 著作 · 2014 · 被引用 81 次 — As previously mentioned, lysis of cells is a common step in most DNA extraction protocols, and it is commonly achieved through the use of ... Read More
The Basics of DNA Extraction – Alaska BioPREP Virtual ... | 合法醫療器材資訊網
The three basic steps of DNA extraction are 1) lysis, 2) precipitation, and 3) purification. Step 1: Lysis In this step, the cell and the nucleus are broken open to​ ... Read More
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