Purification of Specific Cell Population by Fluorescence ... | 合法醫療器材資訊網

由SBasu著作·2010·被引用135次—Fluorescenceactivatedcellsorting(FACS)isasuperiormethodin...Diamond,R.A.Inlivingcolor:Protocolsinflowcytometryandcellsorting.

FACS is a highly sophisticated technique for purifying cell populations of interest, in which a very high purity (95-100%) of the sorted population can be obtained. Therefore, this technique is particularly important for experiments, where high purity is an essential requirement (e.g. microarray analysis). FACS is particularly advantageous over other available methods of purification when a cell population needs to be purified based on a weekly expressed surface marker or when two or more populations need to be purified which have different levels of expression of the same surface marker. For example, the purification of marginal zone B cells (B220+CD21hiCD23int/low) and follicular B cells (B220+CD21int/lowCD23hi) based on expression levels of CD21 and CD23. Another growing application of FACS is single cell sorting allowing the analysis of individual cells (3, 4). FACS is also commonly used...