Blue intensity matters for cell cycle profiling in fluorescence ... | 合法醫療器材資訊網

2019年12月4日 — UV excitable DNA dyes such as DAPI and Hoechst 33342 are normally excited with a UV laser (350-360nm) for cell cycle analysis. There are ... Read More

2019年12月4日 — When using DAPI most antibody fluorophores can be used except for Pacific Blue​. Whilst with PI which emits at 619nm, PE, PerCP or PE-Cy5 or ... Read More

DAPI is commonly used in cell cycle analyses since it preferentially binds dsDNA, when cells are permeabilized, allowing it to intercalate with and saturate the ... Read More

DAPI is commonly used in cell cycle analyses since preferentially binds dsDNA when cells are permeabilized allowing it to saturate the nucleic acids. DAPI will ... Read More

由 A Ferro 著作 · 2017 · 被引用 19 次 — ... to both identify a given DAPI-stained nucleus to its correct cell cycle phase, as well as to determine, with very high probability, true negatives. Read More

由 Z Darzynkiewicz 著作 · 2001 · 被引用 103 次 — Staining the DNA with different fluorescent dyes, propidium iodide or DAPI, is one of the most direct ways of staging the cells based on DNA content. These ... Read More

DAPI staining for cell cycle analysis. Binds preferentially A-T base regions in DNA. Process between 1 - 10 million of cells. 1) Spin the cells at 500g, 5 min, 4°C​. Read More

Cell Cycle Determination Using DAPI – Alcohol Fixation Method. DAPI is commonly used in cell cycle analyses since it preferentially binds dsDNA, when cells ... Read More

由 V Roukos 著作 · 2015 · 被引用 115 次 — On the basis of the DAPI integrated intensity values that define distinct cell cycle phases, as obtained by setting gates on the cell cycle histograms ... Read More