Defective Human Sperm Cells Are Associated with ... | 合法醫療器材資訊網
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由ACassina著作·2015·被引用38次—Infertilityaffectsabout15%ofcouplesofreproductiveage....Briefly,semenaliquots(7μl)wereplacedinCELL-VUdisposablecountingchambers...assistedindistinguishingmitochondrialfromresidual(non-ele
![Defective Human Sperm Cells Are Associated with ...](https://i.imgur.com/6y29Lx7.jpg)
Infertility affects about 15% of couples of reproductive age. The male factor is involved in nearly 50% of infertility cases. Defective human sperm function has been associated with evidence of high levels of reactive oxygen species (ROS) and a resultant loss of fertilizing potential in vivo and in vitro. Analogous to what has been observed in somatic cells, mitochondria are likely the major sources of ROS in sperm cells. In this study, we analyzed mitochondrial function using high-resolution respirometry, ROS production, and footprints of oxidative and nitrative stress processes in intact human sperm cells. We showed that mitochondrial dysfunction (measured through the respiratory control ratio) was correlated with a decrease in human sperm motility. The samples analyzed presented nitro-oxidative modifications of proteins, such as protein 3-nitr...
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