瑞柏生物科技股份有限公司 | 合法醫療器材資訊網

RP05RIPACellLysisBuffer,人氣指數,793...配方透明，1X的RIPA細胞裂解液配方為25mMTris•HClpH7.6,150mMNaCl,1%NP-40,1%sodium ...

RIPA 細胞裂解液對動物細胞膜、細胞質、細胞核成分具有較強的細胞裂解能力，適用於貼附型與懸浮型細胞。經 RIPA 裂解液裂解得到的蛋白樣品可應用於多項實驗操作，包含常規的西方墨點法 (Western Blot)、免疫沉澱法 (Protein immunoprecipitation)、Reporter assay 等等。

產品特點

．應用範圍廣，細胞裂解後之取得的蛋白樣品可相容下游實驗，包含西方墨點法 (Western Blot)、免疫沉澱法(Protein immunoprecipitation)、蛋白質純化等

．配方透明，1X 的 RIPA 細胞裂解液配方為 25mM Tris•HCl pH 7.6, 150mM NaCl, 1%NP-40, 1% sodium deoxycholate, 0.1% SDS

RIPA裂解液 | 合法醫療器材資訊網

Western-blotting | 合法醫療器材資訊網

2019年11月20日 — ... 抑制劑(phosphatase inhibitor)。常見的蛋白質裂解液配方：ACE Biolabs 1X RIPA Lysis Buffer (C1020) 25 mM Tris-HCl pH7.6, 150 mM NaCl, ... Read More

瑞柏生物科技股份有限公司 | 合法醫療器材資訊網

RP05 RIPA Cell Lysis Buffer, 人氣指數, 793 ... 配方透明，1X 的 RIPA 細胞裂解液配方為 25mM Tris•HCl pH 7.6, 150mM NaCl, 1%NP-40, 1% sodium ... Read More

RIPA Buffer Recipe | 合法醫療器材資訊網

RIPA (Radio Immuno Precipitation Assay) buffer recipe & guide from Antibody Genie. RIPA buffer guide includes its uses, materials requires for cell lysis, buffer ... Read More

Protocol - Cell Lysate Preparation | 合法醫療器材資訊網

Materials: Mammalian cells grown in adherent (100 mm dish) or suspension culture; Ice cold RIPA Lysis Buffer; Halt Protease and Phosphatase Inhibitor Single- ... Read More

RIPA Lysis Buffer | 合法醫療器材資訊網

Prepared RIPA buffer should be aliquoted and stored at −20°C. Add protease and/or phosphatase inhibitors to a thawed aliquot before immediate use. Discard ... Read More

蛋白質萃取液 | 合法醫療器材資訊網

Protocol (259 KB). 功能說明. 內容物: 25 mM Tris, 150 mM Sodium Chloride, 1% NP- ... Read More

Western blot sample preparation | 合法醫療器材資訊網

RIPA buffer is useful for whole cell extracts and membrane-bound proteins, and may be preferable to NP-40 or Triton X-100-only buffers for extracting nuclear ... Read More

RIPA Buffer (10X) | 合法醫療器材資訊網

Description: RIPA buffer is used to lyse cells and tissues. 1X RIPA Buffer: 20 mM Tris-HCl (pH 7.5). 150 mM NaCl, 1 mM Na2EDTA. 1 mM EGTA. 1% NP-40. Read More

Best recipe for Ripa buffer? | 合法醫療器材資訊網

2017年10月12日 — The RIPA buffer recipe that is working well for me at the moment: 140 mM NaCl. 25 mM Tris-HCl. 1 mM EDTA. 0.5 mM EGTA. 1 mM PMSF. Read More

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