Principles of immunohistochemistry (IHC) staining | 合法醫療器材資訊網

Immunohistochemistry(IHC)stainingallowsfor2broadclassesofdetection:1)chromogenicand2)fluorescent.Forchromogenicdetection,CSTrecommends ...

Immunostaining uses antibodies to detect an antigen in cells or tissue. The major benefit of immunostaining in immunohistochemistry (IHC) is the ability to see the desired target in a tissue sample while maintaining the spatial context and tissue architecture.

Immunostaining can be performed either directly or indirectly. Conjugating our application-validated antibodies to a number of fluorophores enables direct labeling. To browse our offering of direct conjugates, click here[1].

Often based on antigen expression levels and accessibility, a direct conjugate is not sufficient to visualize the target of interest. In these cases, indirect labeling is employed using a secondary antibody. The preferred method at CST is to utilize highly sensitive, one-step, polymer-based detection reagents specific for rabbit or mouse IgG.