Urease Test- Principle | 合法醫療器材資訊網

2018年6月11日—Therapidureasetest(RUT)isapopulardiagnostictestfordiagnosisofHelicobacterpylori.Itisarapid,cheapandsimpletestthatdetectsthe ...

Urea Agar was developed by Christensen in 1946 for the differentiation of enteric bacilli. The urease test is used to determine the ability of an organism to split urea, through the production of the enzyme urease.

Urea is the product of decarboxylation of amino acids. Hydrolysis of urea produces ammonia and CO2. The formation of ammonia alkalinizes the medium, and the pH shift is detected by the color change of phenol red from light orange at pH 6.8 to magenta (pink) at pH 8.1. Rapid urease-positive organisms turn the entire medium pink within 24 hours.

Weakly positive organisms may take several days, and negative organisms produce no color change or yellow as a result of acid production.

The rapid urease test (RUT) is a popular diagnostic test for di...