Using Seahorse Machine to Measure OCR and ECAR in ... | 合法醫療器材資訊網
由JZhang著作·2019·被引用28次—Specifically,thismachinemeasuresglycolysisbyanalyzingtheextracellularacidificationrate(ECAR)andmeasuresmitochondrialoxidativephosphorylationon ...
A large amount of energy used for nutrient processing and cellular functions is essential for tumorigenesis. Total intracellular adenosine triphosphate (ATP) is mainly generated by glycolysis and mitochondrial oxidative phosphorylation. Here, we provide a protocol for measurements of energy metabolism in cancer cells by using Seahorse XF24 Extracellular Flux analyzer. Specifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the oxygen consumption rate (OCR), through real-time and live cell analysis. This protocol is provided for researchers who are unfamiliar with the method and to aid them in carrying out the technique successfully.
Keywords: ECAR; Energy metabolism; Glycolysis; OCR; Oxidative phosphorylation; Seahorse XF24 Extracellular Flux analyzer.
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Measurement of Oxygen Consumption Rate (OCR) and ... | 合法醫療器材資訊網
Using Seahorse Machine to Measure OCR and ECAR in ... | 合法醫療器材資訊網
Measuring Glycolysis and Oxidative Metabolism in ... | 合法醫療器材資訊網
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【軟体交互作用系統生物電位分析儀】許可證字號:衛署醫器輸字第002278號
器材品名:軟体交互作用系統生物電位分析儀許可證字號:衛署醫器輸字第002278號註銷狀態:已註銷註銷日期:1990/02/26註銷理由:...