Flow Cytometry Protocol for Staining Membrane | 合法醫療器材資訊網

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Immunophenotyping suspended cells based on antigens present on the cell surface is one of the most common uses of flow cytometry. Since cell surface proteins are readily accessible to the antibody, a permeabilization step is not required. By staining cell surface markers, researchers can identify specific cell populations and perform fluorescence-activated cell sorting (FACS).

The following flow cytometry staining protocol has been developed and optimized by R&D Systems Flow Cytometry Laboratory. This protocol is designed for staining of cell surface proteins. It is recommended that experimental conditions, such as antibody concentration, incubation time, and temperature, be optimized for each flow cytometry experiment.

Please read the following flow cytometry staining protocol in its entirety before beginning.