Biuret test | 合法醫療器材資訊網

Thebiurettest,alsoknownasPiotrowski'stest,isachemicaltestusedfordetectingthepresenceofpeptidebonds.Inthepresenceofpeptides,acopper(II)ion ...

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Chemical test for detecting peptide bonds

In chemistry, the Biuret test (IPA: , [1]), also known as Piotrowskis test, is a chemical test used for detecting the presence of at least two peptide bonds in a molecule. In the presence of peptides, a copper(II) ion forms mauve-colored coordination complexes in an alkaline solution. The reaction was first observed in 1833;[2] In Poland, the biuret test is also known as Piotrowskis test in honor of the Polish physiologist Gustaw Piotrowski [pl] who independently rediscovered it in 1857.[3] Several variants on the test have been developed, such as the BCA test and the Modified Lowry test.[4]

The biuret reaction can be used to assess the concentration of pr...