Oxidase Test- Principle, Uses, Procedure | 合法醫療器材資訊網

2018年9月27日—Theoxidasetestdetectsthepresenceofacytochromeoxidasesystemthatwillcatalysethetransportofelectronsbetweenelectrondonorsin ...

The oxidase test detects the presence of a cytochrome oxidase system that will catalyse the transport of electrons between electron donors in the bacteria and a redox dye- tetramethyl-p-phenylene-diamine. The dye is reduced to deep purple color. This test is used to assist in the identification of Pseudomonas, Neisseria, Alcaligens, Aeromonas, Campylobacter, Vibrio, Brucella and Pasteurella, all of which produce the enzyme cytochrome oxidase.

A number of reagents can be used for this test.

          1% tetra-methyl-p-phenylenediamine dihydrochloride, in water

         1% dimethyl-p-phenylenediamine dihydrochloride, in water

          1% α-naphthol in 95% ethan...