Flow Cytometry Troubleshooting Guide | 合法醫療器材資訊網

70%ethanol(ice-cold)cansometimesbeusedasanalternativemethodoffixation,particularlywhenperformingcellcycleanalysis.Notethatfixationcan ...

Weak or no fluorescence signal detected by flow cytometer.

Target expression was insufficiently induced by treatment.

Optimize treatment conditions for successful and measurable induction of each target.

If using PBMCs, avoid frozen samples. Isolate fresh cells whenever possible.

Include the appropriate controls:

Inadequate fixation and/or permeabilization of sample.

If the target of interest is intracellular make sure that the appropriate fixation and permeabilization protocol is used. Different forms of fixation and permeabilization may be required, depending on the target and whether surface marker staining is carried out at the same time. Formaldeh...