Eosin methylene blue | 合法醫療器材資訊網

Eosinmethyleneblue(EMB,alsoknownasLevine'sformulation)isaselectivestainforGram-negativebacteria....EMBcontainsdyesthataretoxictoGram- ...

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Biological stain

Eosin methylene blue (EMB, also known as "Levines formulation") is a selective stain for Gram-negative bacteria.[1] EMB contains dyes that are toxic to Gram-positive bacteria. EMB is the selective and differential medium for coliforms. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1. EMB is a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that do not (e.g., Salmonella, Shigella).[2] Organisms that ferment lactose display "nucleated colonies"—colonies with dark centers.[3]

This medium is important in medical laboratories by di...