Bacteroides Bile Esculin and Laked Kanamycin Vancomycin ... | 合法醫療器材資訊網

BacteroidesBileEsculin(BBE)agarisanenriched,selective,anddifferentialmediumusedfortheisolationandpresumptiveidentificationofobligatelyanaerobic ...

BBE agar contains casein, soy peptones, hemin, and vitamin K1 as the nutritive base of the media. Selective agents include gentamicin, which inhibits facultative anaerobes, and bile, which inhibits most gram-positive bacteria and anaerobic organisms other than the B. fragilis group. The addition of esculin and ferric ammonium citrate to the media permits the recognition of esculin hydrolysis by the organisms. The result is a brown to black coloration around the esculin-positive colonies. Bilophila species usually produce black-centered colonies on BBE due to hydrogen sulfide production. Hemin is added as a growth factor and allows testing of catalase production. This media is prepared, stored, and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.