A fluorescence immunoassay for a rapid detection of Listeria ... | 合法醫療器材資訊網

由ACapo著作·2020—ToreducethedetectiontimeofListeriamonocytogenesinfood,inthisworkwedevelopedafluorescencesandwichimmunoassaybasedon ...

All the used reagents were chosen with a purity grade ≥ 98.0%. 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide (EDC), N-Hydroxysuccinimide, N,N-Dimethylformamide, bovine serum albumin (BSA; fraction V), non-fat dried milk, gelatin from cold-water fish skin, tripolyphosphate (TPP), and 3,5-tetramethylbenzidine (TMB) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Microplates (96-well) Nunc LockWell MaxiSorp C8 form, F16MOD black 96 microplates and the fluorescent probe (AF488) Alexa Fluor 488 NHS Ester (Succinimidyl Ester) purchased from Thermo Fisher SCIENTIFIC (Waltham, Massachusetts, USA). Sephadex G-25 Fine, Polyvinylidene Difluoride (PVDF) transfer membranes 0.45 nm and reagents utilized for Western blot experiments acquired from Amersham Biosciences (GE Healthcare Switzerland). Goat polyclonal to rabbit and mouse IgG—HRP conjugate (secondary antibody) was from abcam (Cambridge, U...