RT-PCR: One-Step vs. Two-Step | 合法醫療器材資訊網
![RT-PCR: One-Step vs. Two-Step](https://i.imgur.com/6y29Lx7.jpg)
Discoverthedifferencebetweenone-stepandtwo-stepRT-PCR.Learnwhenitisbesttouseone-stepRT-PCRandwhatapplicationscallfortwo-stepRT-PCR....RT-PCRSystem.Format,Stand-aloneenzyme.Ordernow,cDNAsynthesiskit
![RT-PCR: One-Step vs. Two-Step](https://i.imgur.com/6y29Lx7.jpg)
RT-PCR can be performed in a one-step or a two-step assay. One-step assays combine reverse transcription and PCR in a single tube and buffer, using a reverse transcriptase along with a DNA polymerase. One-step RT-PCR only utilizes sequence-specific primers. In two-step assays, the reverse transcription and PCR steps are performed in separate tubes, with different optimized buffers, reaction conditions, and priming strategies.
Take a look: One-step vs two-step RT-PCRFigure 1. One-step vs. two-step RT-qPCR.
What is one-step RT-PCR?As the name implies, one-step RT-PCR combines first-strand cDNA synthesis (RT) and subsequent PCR in a single reaction tube. This reaction setup can help simplify your workflow, reduces variation, and minimizes possible contamination. One-step RT-PCR allows easy...
One-Step RT | 合法醫療器材資訊網
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