Stain,Buffer,(FBS),RUO - 554656 | 合法醫療器材資訊網

StainBuffer(FBS)canbeusedfortheimmunofluorescentstainingofsingle-cellsuspensionspreparedfromeitherlymphoidtissues,bonemarrow,peripheral ...

Flow Cytometry (Direct immunofluorescence staining):

1.   Prepare single-cell suspensions from either lymphoid tissue, bone marrow, peripheral blood or cell cultures using standard protocols.

2.   Wash the cells twice in cold Stain Buffer (FBS) and pellet the cells by centrifugation (e.g., 300 x g at 4°C). Resuspend the cell pellet with cold Stain Buffer (FBS) to a final concentration of 2 x 10e7 cells/ml.

3.   Distribute 50 µl aliquots of the cell suspension (10e6 cells) to either tubes or the round-bottomed wells of microwell plates.

4.   Dilute fluorescent antibodies to their predetermined optimal concentrations in Stain Buffer (FBS) and add small aliquots (e.g., 10 µl) of the diluted antibodies to the tubes or microwells that contain the target cell suspensions.  Incubate for 20 minutes on ice protected ...